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Nonsterile Paraformaldehyde, 4% Protocol (a) Remove medium and rinse 3 times with phosphate-buffered saline (PBSA). Leave the third addition of PBSA on the cultures for 3 5 min. (b) Remove PBSA and add 1 ml 0.1 mM EDTA per well or dish. Incubate at 37 C for 10 20 min, checking cells at 5-min intervals to see whether they are ready for the addition of trypsin-EDTA, as indicated by retraction of the cells (cells should not be completely round). If the cells are being released on days 1 or 2 of culture, this step can be skipped. (c) Remove EDTA and add 475 l trypsin-EDTA per well and incubate at 37 C for 5 to 7 min. (d) Return plate to room temperature. Agitate by hand if any cells remain adherent. (e) Add 25 l FBS and 25 l trypsin inhibitor. Swirl or rock plate to mix. (f) With a Pasteur pipette, combine suspensions from 3 wells (of a 6-well plate) into 1 well. Gently ush empty wells once or twice with the cell suspension. (g) Transfer cell suspension to a 12 75-mm polypropylene tube. (h) Rinse empty wells with a total volume of 500 ml PBSA and add the rinse to the polypropylene tube. (i) Cells may be counted at this point. (j) Bring suspension to a concentration of 0.5% paraformaldehyde with the addition of a suitable volume of 4% paraformaldehyde. (k) Invert the tube several times to mix contents, and store at 4 C until the day of staining.

Finally, we need to describe how to delete database records. Deleting records from a Windows CE database is done with the CeDeleteRecord function: CeDeleteRecord(hDatabase, oidRecord); hDatabase is the handle of the open database from which the record is to be removed. oidRecord is the CEOID identifying the record to delete. CeDeleteRecord returns TRUE if the specified record is successfully deleted from the database. Otherwise the function returns FALSE. In this case, an application can call GetLastError for more information about why the delete operation failed.

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the evil involved in bystanders who allow genocides to occur Stohl (1987) showed that nations are typically bystanders, and this is re ected in the minimal news devoted to accounts of genocidal actions However, the judgment that genocide is evil is re ected by the development of an international norm against genocide that may eventually be enforced by the establishment of a permanent international court Without the concept of evil it might be easy for people to avoid making judgments that need to be made In this regard, Miller, Gordon, and Buddie (1999) have evinced concern that situational explanations of criminal actions may result in persons condoning such actions They demonstrate that when persons make judgments after situational explanations, they have less unfavorable attitudes and punitive responses toward the perpetrator.

Fig. 4.16 A rat can be trained to swim from a point (X) to a submerged platform along a direct route (a). If the rat is placed into the water at a novel position (b), it will still swim directly to the platform. If the platform is moved (c) the rat will search the area in which it had been trained to expect the platform, before eventually locating its new position. (Adapted from Morris, R.G.M. (1981) Spatial localization does not require the presence of local cue. Learning and Motivation, 12, 239 60. Reproduced with permission of Elsevier.)

Table 24.5. Bioactive peptides derived from meat and meat-related proteins Bioactivity Antihypertensive (ACE inhibitory) Protein source Chicken muscle Chicken muscle creatine kinase Chicken muscle aldolase Chicken muscle Porcine muscle actin Porcine myosin Porcine myosin Chicken muscle myosin Bovine muscle Chicken muscle creatine kinase Fermented pork myosin Chicken muscle actin Porcine muscle troponin C Chicken muscle collagen Antioxidative Porcine Porcine Porcine Porcine muscle muscle muscle muscle actin Sequencea IKW LKA LKP LAP VWI ITTNP MNPPK FQKPKR VLAQYK FKGRYYP VFPMNPPK IVGRPRHQG RMLGQTPTK GFXGTXGLXGF VW DLYA SLYA DLQEKLE VVYPWTQRF LVVYPWTQRF KGDEESLA APPPPAEVHEV References Fujita et al., 2000 Fujita et al., 2000 Fujita et al., 2000 Fujita et al., 2000 Arihara et al., 2005a Nakashima et al., 2002 Nakashima et al., 2002 Fujita et al., 2000 Jang & Lee, 2005 Fujita et al., 2000 Arihara et al., 2004 Fujita et al., 2000 Katayama et al., 2003b; 2004 Saiga et al., 2003a Arihara et al., 2005b Arihara et al., 2005b Arihara et al., 2005b Saiga et al., 2003b Zhao, et al., 1997 Zhao, et al., 1997 Hau, et al., 1997 Okumura et al., 2004