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Transfection of the telomerase gene hTRT into normal cells with a nite life span allows a small proportion of the cells to become immortal [Bodnar et al, 1998; Protocol 182, Freshney, 2005], although this probably involves deletion or inactivation of other genes such as p53 and myc [Cerni, 2000] 42 Growth Cycle Each time that a cell line is subcultured it will grow back to the cell density that existed before subculture (within the limits of its nite life span) This process can be described by plotting a growth curve from samples taken at intervals throughout the growth cycle (Fig 15), which shows that the cells enter a latent period of no growth, called the lag period, immediately after reseeding.

the calculated syndrome, according to equation (31) The following is an example based on the cyclic code Ccyc (7, 4) Example 35: The cyclic linear block code Ccyc (7, 4) generated by the polynomial g(X ) = 1 + X + X 3 , which has a minimum Hamming distance dmin = 3, can correct any single error pattern There are seven patterns of this kind Table 33 shows single error patterns and their corresponding syndromes for this cyclic linear block code The all-zero pattern corresponds to the decoding of a code polynomial (ie, the no-error case) As in the block code case, once the syndrome has been calculated and the corresponding error pattern found from the table, then the error-correction procedure is to add the error pattern to the received vector.

where n is equal to the number of data points (i.e., the group n). By taking the arccosine of cos ( M i )/n, the variance can be expressed in degrees, which is generally more easily interpreted. In our example, the circular variance is equal to .19, or, in degrees, a dispersion of 36 . Finally,

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This period lasts from a few hours up to 48 h, but is usually around 12 24 h, and allows the cells to recover from trypsinization, reconstruct their cytoskeleton, secrete matrix to aid attachment, and spread out on the substrate, enabling them to reenter cell cycle They then enter exponential growth in what is known as the log phase, during which the cell population doubles over a de nable period, known as the doubling time and characteristic for each cell line As the cell population becomes crowded when all of the substrate is occupied, the cells become packed, spread less on the substrate, and eventually withdraw from the cell cycle They then enter the plateau or stationary phase, where the growth fraction drops to close to zero Some cells may.

Of course, if an error-pattern polynomial has the form of a code polynomial, then it will be undetectable and therefore uncorrectable, as both the syndrome and the error pattern will be null vectors Theorem 31 is the basis of several other decoding algorithms for cyclic codes [1] One of them is the Meggitt algorithm [5] This decoder is based on the relationship between the cyclically shifted versions of a given codeword and their corresponding shifts in the received vector It operates in a bit-by-bit mode, so that it rst determines if the most signi cant bit contains an error or not, and then cyclically shifts the received vector and the corresponding syndrome in order to analyse the following bits of the received vector.