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1. Position the cursor where you want the applet to originate and choose Insert Media Applet. You can also click the Insert Applet button from the Media group on the Common category of the Insert panel. The Insert Applet dialog box opens. 2. From the Select File dialog box, enter the path to your class file in the File Name text box or click the Browse button to locate the file. An Applet object placeholder appears in the Document window. In the Applet Property inspector (see Figure 5-33), the selected source file appears in the Code text box, and the folder appears in the Base text box.

10.68 In the network shown in Fig. PI 0.68, detennine the value of the load impedance for maximum power transfer.

(32.25) where we are defining the scalar (32.26) Finally, if we multiply both sides of (32.20) by [u a] from the left and col{u*, a*}from the right, and use the definitions of {r,,-y, 6 } in (32.6), (32.12), and (32.18), we find that (32.27)

/***

i, (t )

e,(i) = ui.iiri-1

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lysins interact with those components of the cell wall that are necessary for bacterial viability (Borysowski et al 2006; Fischetti 2008) For instance, the receptor for S pneumoniae-speci c lysins is choline, an essential component of the pneumococcal cell wall In fact, in none of the hitherto conducted studies have any lysin-resistant bacteria been identi ed (Loef er et al 2001) In two separate studies, both S pneumoniae and B cereus failed to develop resistance to lysin even following repeated exposure to low doses of enzyme (the same results were obtained in experiments carried out on solid media and in liquid cultures; Loef er et al 2001; Schuch et al 2002) Furthermore, B cereus remained sensitive to lysin even after exposure to mutagens that rapidly induced mutations resulting in resistance to novobiocin and streptomycin (Schuch et al 2002).

On the other hand, it was found that bacteria can be less susceptible to lysins during the stationary phase, probably owing to some changes in the cell wall composition (Borysowski et al 2006) While no cases of bacterial resistance to lysins have been reported as yet, four mechanisms inducing resistance to lysostaphin have been identi ed (Shaw et al 2005; Gr ndling et al 2006; Kusuma et al 2007) These are described in more detail in 7 Interestingly, in some cases the development of resistance to lysostaphin in MRSA results in an increase in susceptibility to methicillin and other antibiotics as well as a reduction in bacterial tness and virulence (Kusuma et al 2007) Moreover, two general mechanisms mediating bacterial resistance to lysozyme have been reported.

You can convert any paragraph or line into a heading through the Format options in the Text Property inspector HTML tab.

The rst is based on the modi cations of some peptidoglycan components (either O-acetylation of N-acetylmuramic acid residues by O-acetyltransferase or deacetylation of N-acetylglucosamine residues by N-acetylglucosamine deacetylase; Vollmer and Tomasz 2000; Bera et al 2005) Such modi ed residues restrict the access of lysozyme to its substrate The other mechanism involves the production of a lysozyme inhibitor (Binks et al 2005) 62 Gram-negative Bacteria While in Gram-positive bacteria peptidoglycan is easily accessible to recombinant lytic enzymes from outside the cell, in Gram-negative bacteria it is protected by the outer membrane that is impermeable to macromolecules (Vaara 1992) Therefore, Gram-negative bacteria are essentially resistant to lytic enzymes However, the results of some studies indicate that these bacteria can also be killed by recombinant lytic enzymes.

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Virtually all 10/100 switches are autosensing, meaning that each port automatically adjusts itself for 10 or 100 mbps operation, depending on the device connected to that port. This is an important feature if you plan to have a mix of 10 and 100 mbps devices on your LAN. Figure 5-8 shows the front and rear views of a 16-port switch.

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If you select one or more subpaths, the Fill Path command changes to Fill Subpaths, enabling you to fill only the selected subpaths. The fill path icon also affects only the selected subpaths.

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